In 2009, the WHO produced a Human Papillomavirus laboratory manual Human papillomavirus laboratory manual, First Edition, 2009 to assist in establishing the laboratory support required for implementation and monitoring of human papillomavirus (HPV) vaccination programmes. Since then, both HPV vaccination and HPV-based cervical screening have become key pillars of the WHO strategy for global elimination of cervical cancer.
The Global Network of National HPV Reference Laboratories has therefore produced an updated HPV Laboratory e-Manual as a comprehensive and interactive guide for professionals working with HPV research and HPV-based cancer control. The manual covers essential topics, including:
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Introduction
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HPV Types
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Laboratory Quality Assurance
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Collection and Handling of Specimens for HPV Testing
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Nucleic Acid Extraction
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HPV Detection and Typing
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HPV Serology – ELISA
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HPV International Standards and Secondary Standards
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Data Management
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International and National HPV Reference Centers
Each section is designed to provide up-to-date methodologies, protocols, and best practices to ensure high-quality results in HPV testing and research. With interactive features and real-time updates, this manual enhances laboratory workflows, supports precision, and keeps you informed of the latest advancements in the field.
Editors
The editors of the E-laboratory manual include Dr Laila Sara Arroyo Mühr and Professor Joakim Dillner (International HPV Reference Center, Center for Cervical Cancer Elimination, Karolinska Institutet, Sweden) together with Dr. Troy Querec and Dr Elizabeth R Unger (Global HPV Reference Laboratory (GRL), Centers for Disease Control and Prevention, USA).
Contributors
International HPV Reference Center, Sweden
HPV Laboratory at Centers for Disease Control and Prevention, USA
National Reference Laboratory, Scotland
National Reference Laboratory, Argentina
National Reference Laboratory, France
National Institute of Biological Standards and Control, United Kingdom
National Reference Laboratory, Australia
National Reference Laboratory, Japan
National Reference Laboratory, Slovenia
National Reference Laboratory, Norway
National Reference Laboratory, Belgium
National Reference Laboratory, Germany
National Reference Laboratory, France
National Reference Laboratory, Sweden
In Chapter 10, you can see the members and contacts for each National Reference Laboratory.
Acknowledgments
We would like to express our gratitude to the Global Network of National Human Papillomavirus Reference Laboratories (HPV LabNet) for their valuable input and efforts in making this manual possible. We also extend our thanks to the following expert for providing helpful comments and advice on this document: Partha Basu (International Agency for Research on Cancer, IARC, Lyon).
Abbreviations
Click here to view the full list of abbreviations.