Abbreviations and Acronyms

A/A: antibiotic-antimycotic

aHSIL: anal high grade squamous intraepithelial lesion

ANCHOR: anal cancer HSIL (high grade squamous intraepithelial lesion) outcomes research

BLAST: basic local alignment search tool

BSC: biological safety cabinet

BSL: biosafety level

CDC: Center for Disease Control and Prevention

cDNA: complementary DNA

CAP: College of American Pathologists

CAPA: corrective and preventive action

ccfDNA: cell-free DNA

CIA: chemiluminescent Immunoassay

CIN: cervical intraepithelial neoplasia

CIN2+: cervical intraepithelial neoplasia grade 2 or higher

CP: control positive

CPD: continued professional development

Cq: cycle validation

CsCl: caesium chloride

Ct: cycle threshold

CV: coefficient of variance

DAMA: Data management association

DEPC: diethylpyrocarbonate

DBPS: Duulbecco´s phosphate buffered saline

DMEM-PF: phenol red-free Dulbecco’s modified Eagle Medium

DNA: deoxyribonucleic acid

dNTPs: deoxynucleoside triphosphates

dPCR: digital PCR

dsDNA: double stranded DNA

ECBS: Expert Committee on Biological Standardization

EDTA: ethylene-diamine-tetra-acetic acid E1-E7 early proteins of HPV numbered E1–E7 (however no E3)

EIA: enzyme immunoassay

ELISA: enzyme-linked immunosorbent assay

ENC: extraction negative congrol

EQA: external quality assessment

eWGS: enriched whole gnome sequencing

FBS: fetal bovine serum

FFPE: formalin fixed paraffin embedded

GE: genome equivalents

GLUT: Glutamax I

HBV: hepatitis B virus

HC2: hybrid capture 2 (Digene®, Qiagen)

HCV: hepatitis C virus

H&E: hematoxylin and eosin

HIV: human immunodeficiency virus

HPV: human papillomavirus

HPV: LabNet Global network of national HPV reference laboratories

HPVRG: human papillomavirus research group

HR: high risk

HRP: horseradish peroxidase

HSIL: high grade squamous intraepithelial lesion

IARC: International Agency for Research on Cancer

IATA: International Aviation and Transportation Administration

ICH: International Committee on Harmonization

ICTV: International Committee on Taxonomy of Viruses

ID: identification

Ig: immunoglobulin

IgG: immunoglobulin G

ILS: interlaboratory schemes

IHRC: International HPV Reference Center

IQC: internal quality control

IRB: internal review board

IS: international standard

iSNVs: intrahost single nucleotide variants

IT information technology

IU: international unit

IVD: in vitro diagnostic medical device

IVDR: in vitro diagnostic medical device regulation

kDa: kilodalton

L: litre

L1, L2: late proteins of HPV, major (L1) and minor (L2) proteins of capsid lab laboratory

LAMP: loop-mediated isothermal amplification

LBC: liquid based cytology

LDT: laboratory developed test

LIMS: laboratory information systems

LQA: laboratory quality assurance

LR: low risk

LSIL: low grade squamous intraepithelial lesion

M: molar

mg: milligram

MGP: modified general primers

min: minute

MiQE: minimum information for publication of quantitative real-time PCR

mL: millilitre

mM: millimetre

MSD: meso scale discovery

uL: microlitre

mRNA: messenger RNA

MS mass spectrometry

MSDS: material safety data sheet

NCBI: National Center for Biotechnology Information

NEAA: non-essential amino acids

NGS :next generation sequencing

NIBSC: National Institute for Biological Standards and Control

NIH: National Institutes of Health

nm: nanometer (10-9 metre)

NRL: national reference laboratory

NTC: no template control

OD: optical density

ORF: open reading frame

PBS: phosphate buffered saline

PAHO: panamerican health organization

PAVE: Papillomavirus Episteme

PCR: polymerase chain reaction

PDCA: Plan-Do-Check act

pg: picogram

PLL: parallel line

PsV: pseudovirion

QA: quality assurance

QC: quality control

QMS: quality management system

qPCR: real time PCR

RNA: ribonucleic acid

RPA: recombinase polymerase amplification

rtPCR: reverse transtription RCR

SD: standard deviation

SHPVRL: Scottish HPV reference laboratory

SOP: standard operating procedure

SPF: short PCR fragment

STM: specimen transport media (Qiagen)

TameSeq: tagmentation assisted multiplex PCR enrichment sequencing

TBE: (buffer) Tris Borate EDTA

TE: (buffer) Tris EDTA

Tm: melting temperature

UN: United Nations

VALGENT: validation of HPV genotyping tests

VLP: virus-like particle

WHO: World Health Organization